Synaptophysin is an antibody that recognizes proteins with a molecular weight of 38kDa. It can help to label normal neuroendocrine cells of the carotid body, pituitary gland, skin, adrenal medulla, thyroid, pancreas, lung, and gastrointestinal mucosa. It can also label Paneth’s cells of the gastrointestinal tract and gastric parietal cells. Likewise, it can label neurons in the spinal cord, retina, and brain. The anti-synaptophysin antibody can react with neuroendocrine neoplasms of epithelial and neural types, such as ganglioneuroblastomas, neuroblastomas, pheo-chromocytomas, non-chromaffin paragangliomas, ganglioneuromas, and chromaffin.
Synaptophysin has no known clone, and the immunogen is the synthetic peptide of the human antibody of the same name. The isotype is the Rabbit IgG, and it has an undetermined epitope with a molecular weight of 38kDa.
This antibody can be used in Immunohistochemistry applications. To prepare your specimen, you should choose paraffin-embedded or Formalin-fixed tissues. Deparaffinized slides should be used, and you can choose graded alcohols or xylene.
If you choose the concentrated formula of the product, you should dilute the antibody using a ratio of 1:300, though this is an estimate and your dilution needs may be different if you have various methods or protocols. You will also find a pre-diluted product that works well for ratios of 1:300 only.
To retrieve your antigen, you should boil the tissue sections for 10 minutes in a 10mM citrate buffer with a pH of 6.0. It should be allowed to cool to room temperature for 20 minutes. Then, you should incubate for 10 minutes while still at room temperature.
The positive control for this antibody includes the pheochromocytoma and pancreas with cellular localization occurring in the cytoplasm.
Synaptophysin is an excellent antibody to use for detecting a variety of things in humans. Visit Spring Bioscience today for more information.
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